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occludin s490 phosphorylation regulates vascular

occludin s490 phosphorylation regulates vascular

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12345NextThe occludin and ZO-1 complex,defined by small angle X

Jul 03,2012·Y474 phosphorylation is associated with binding p85 of phosphatidyl inositol 3-kinase at the leading edge of migrating cells while S490 and S508 are associated with vascular permeability after VEGF treatment or HIV encephalitis .Our results suggest a role for S471 phosphorylationBrain endothelial cell junctions after cerebral hemorrhage Liu,X,Dreffs,A,Diaz-Coranguez,M Occludin S490 phosphorylation regulates vascular endothelial growth factor-induced retinal neovascularization.Am J Pathol 2016; 186 2486 2499.Google Scholar Crossref Medline

Cited by 15Publish Year 2016Author Xuwen Liu,Alyssa Dreffs,Monica Díaz-Coránguez,E.Aaron Runkle,Thomas W.Gardner,Vince A.ChiodoImages of Occludin S490 Phosphorylation Regulates Vascular

imagesOccludin Phosphorylation and Ubiquitination Regulate Tight Phosphorylation of occludin on Ser-490 and ubiquitination regulate VEGF-induced vascular endothelial permeability.A,diffusive flux (P o) of 70-kDa-dextran was evaluated in the absence or presence of VEGF (50 ng/ml),after the transfection of each occludin mutant.VEGF-induced hyperpermeability was inhibited by S490AOcc,whereas Occ-Ub and S490A-Ub increased the tracerCited by 348Publish Year 2009Author Tomoaki Murakami,Edward A.Felinski,David A.AntonettiMónica Díaz-Coránguez's research works University of Vascular endothelial growth factor (VEGF) induces phosphorylation of occludin at S490,which is required for VEGF-induced endothelial permeability.Herein,we demonstrate thatCited by 348Publish Year 2009Author Tomoaki Murakami,Edward A.Felinski,David A.AntonettiOccludin S490 Phosphorylation Regulates Vascular Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth FactorInduced Retinal Neovascularization By Xuwen Liu,Alyssa Dreffs,Monica Díaz-Coránguez,E.Aaron Runkle,Thomas W.Gardner,Vince A.Chiodo,William W.Hauswirth and David A.Antonetti

Complex Phenotype of Mice Lacking Occludin,a Component

Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth FactorInduced Retinal Neovascularization The American Journal of Pathology,Vol.186,No.9 Highly Conserved Testicular Localization of Claudin-11 in Normal and Impaired SpermatogenesisCyclic StrainMediated Regulation of Vascular Endothelial Shear stress regulates occludin content and phosphorylation.Am J Physiol Heart Circ Physiol.2001; 281 H105H113.Crossref Medline Google Scholar; 21 Conklin BS,Zhong DS,Zhao W,Lin PH,Chen C.Shear stress regulates occludin and VEGF expression in porcine arterial endothelial cells.J Surg Res.2002; 102 1321.Crossref Medline Google Dr.Thomas Gardner,MD Ann Arbor,MI OphthalmologyOccludin S490 Phosphorylation Regulates Vascular Endothelial Growth FactorInduced Retinal Neovascularization Thomas Gardner,MD> ;The American Journal

Endothelial cell junctions and the regulation of vascular

regulating junctional protein phosphorylation is underscored by the observation that certain phosphatases constitutively associate with cellcell junctions in quiescent ECs,presumably to maintain the low level of phosphorylation required for junctional integrity [22].Occludin phosphorylation is a major mechanism for regu-lating TJ permeability.Endothelial cell junctions and the regulation of vascular regulating junctional protein phosphorylation is underscored by the observation that certain phosphatases constitutively associate with cellcell junctions in quiescent ECs,presumably to maintain the low level of phosphorylation required for junctional integrity [22].Occludin phosphorylation is a major mechanism for regu-lating TJ permeability.IschemiaReperfusion Injury Induces Occludin Ischemiareperfusion injury also rapidly induced the activation and phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) at tyrosine 1175.Blocking vascular endothelial growth factor (VEGF) function by intravitreal injection of bevacizumab prevented VEGFR-2 activation,occludin phosphorylation,and vascular permeability.

Ischemiareperfusion injury induces occludin

Ischemiareperfusion injury also rapidly induced the activation and phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) at tyrosine 1175.Blocking vascular endothelial growth factor (VEGF) function by intravitreal injection of bevacizumab prevented VEGFR-2 activation,occludin phosphorylation,and vascular permeability.Mónica Díaz-Coránguez's research works University of Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth FactorInduced Retinal Neovascularization.Article. (VEGF) induces phosphorylation of occludin at S490Mechanisms of Retinal Vascular Permeability in Diabetes Liu,Xuwen; Dreffs,Alyssa; Díaz-Coránguez,Monica et al.(2016) Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth Factor-Induced Retinal Neovascularization.Am

Mechanisms of Retinal Vascular Permeability in Diabetes

Published and preliminary data also reveal that occludin phosphorylation contributes to growth control and angiogenesis.Thus,occludin phosphorylation downstream of VEGF activation contributes to both vascular permeability and angiogenesis suggesting an important roleMichigan Diabetes Research Center Microscopy,Imaging Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth Factor-Induced Retinal Neovascularization AUTHORS Liu X,Dreffs A,Díaz-Coránguez M,Runkle EA,Gardner TW,Chiodo VA,Hauswirth WW,Antonetti DA.Modulation of the p75 neurotrophin receptor using LM11ANext we examined phosphorylation of occludin at serine 490 (p-occludin S490),a perquisite for ubiquitination and degradation of the tight junction proteins.Western blot analysis revealed that treatment of HRE cells with hm-proNGF induced significant activation of p-occludin S490 (1.7-fold) and that this activation was blocked by p75 NTR

ORIGINAL ARTICLE Protein Kinase Cb Phosphorylates

demonstrated a number of phosphorylation sites on occludin that regulate barrier properties.Phosphorylation of threo-nines 403/404 by PKCh and threonines 424/438 by PKCz promotes occludin localization to TJ (16,17).Meanwhile,Src-induced tyrosine phosphorylation on Tyr398 and Tyr402 regulates hydrogen peroxideinduced alterations to theOccludin OCEL-domain interactions are required for Although the significance of this observation is not clear,it is interesting that the positively charged OCEL face and S490,whose phosphorylation and ubiquitination have been linked to vascular endothelial growth factorinduced occludin endocytosis in endothelial cells (Murakami et al.,2009,2012) are adjacent to residues 485 and 488 but at some distance from the K433 site.Thus K433 may define a secondOccludin Phosphorylation and Ubiquitination Regulate Tight Here we demonstrate that occludin phosphorylation and ubiquitination regulate VEGF-induced TJ protein trafficking and concomitant vascular permeability.VEGF treatment induced TJ fragmentation and occludin trafficking from the cell border to early and late endosomes,concomitant with increased occludin phosphorylation on Ser-490 and ubiquitination.

Occludin Phosphorylation and Ubiquitination Regulate Tight

Jul 31,2009·Phosphorylation and Ubiquitination of Occludin Regulates Vascular Permeability The contribution of occludin phosphorylation and ubiquitination to endothelial permeability of 70-kDa-rhodamine B isothiocyanate dextran and transendothelial electrical resistance were evaluated in monolayers of BREC transfected with either WtOcc or S490AOcc.Occludin Phosphorylation and Ubiquitination RegulateHere we demonstrate that occludin phosphorylation and ubiquitination regulate VEGF-induced TJ protein trafficking and concomitant vascular permeability.VEGF treatment induced TJ fragmentation and occludin trafficking from the cell border to early and late endosomes,concomitant with increased occludin phosphorylation on Ser-490 and ubiquitination.Occludin S408 phosphorylation regulates tight junction Similarly,phosphorylation of the more distal S490,which is located within the OCEL domain,attenuates the interaction between occludin and ZO-1 (Sundstrom et al.,2009) and is associated with occludin endocytosis and barrier loss (Murakami et al.,2009).Thus,although hyperphosphorylation is associated with occludin trafficking to the TJ

Occludin S408 phosphorylation regulates tight junction

Similarly,phosphorylation of the more distal S490,which is located within the OCEL domain,attenuates the interaction between occludin and ZO-1 (Sundstrom et al.,2009) and is associated with occludin endocytosis and barrier loss (Murakami et al.,2009).Thus,although hyperphosphorylation is associated with occludin trafficking to the TJ Plateletderived growth factor mediates tight junction Oct 10,2002·PDGF altered the cellular distribution of occludin and ZO1 from the cell border to the cytoplasm and increased permeability to 70 kDa dextran in a concentrationdependent manner. Vince A.Chiodo,William W.Hauswirth,David A.Antonetti,Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth FactorInduced Retinal Post-translational modifications of tight junction Sep 01,2020·Phosphorylation on serine S490 attenuated the interaction with ZO-1 as shown by pull-down assays using BREC lysates and human wildtype or mutant occludin with serine S490 mutated to aspartate .As suggested by the authors,phosphorylation on S490 leads to dissociation from ZO-1,which in turn seemed to liberate and expose occludin to the Itch

Post-translational modifications of tight junction

Sep 01,2020·Phosphorylation on serine S490 attenuated the interaction with ZO-1 as shown by pull-down assays using BREC lysates and human wildtype or mutant occludin with serine S490 mutated to aspartate .As suggested by the authors,phosphorylation on S490 leads to dissociation from ZO-1,which in turn seemed to liberate and expose occludin to the Itch ligase.Previous123456NextUbiquitin-based modifications in endothelial cellcell PKC-mediated phosphorylation of occludin at S490 (Murakami et al.,2012) and its subsequent ubiquitylation by the E3 ligase Itch have been linked to vascular endothelial growth factor (VEGF)-induced permeability in primary bovine retinal ECs (Murakami et al.,2012).Protein Kinase C Phosphorylates Occludin Regulating Tight Here we report that VEGF-induced PKC activation regulates occludin phosphorylation on Ser490 and allows ubiquitination of occludin leading to TJ disruption and increased vascular permeability in retinal endothelial cells.

Protein Kinase C Phosphorylates Occludin Regulating Tight

PKC and occludin phosphorylation/ubiquitination regulates VEGF-induced endothelial permeability.The data shown above suggest that PKC is an upstream regulator for occludin phosphorylation and ubiquitination,posttranslational modifications that have been shown to regulate TJ trafficking and permeability .The requirement for occludin Ser490 phosphorylation in PKC-induced vascularPublications UM Neuroscience Graduate ProgramInhibition of Atypical Protein Kinase C Reduces Inflammation-Induced Retinal Vascular Permeability.David Antonetti.Tuesday,October 2,2018. Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth Factor-Induced Retinal Neovascularization.Thomas Gardner.Thursday,September 1,Publications UM Neuroscience Graduate ProgramOccludin S490 Phosphorylation Regulates Vascular Endothelial Growth Factor-Induced Retinal Neovascularization.

Regulatory mechanisms,prophylaxis and treatment of

Mar 15,2017·Vascular leakage,or increased vascular permeability,is a common but important pathological process for various critical diseases,including severe trauma,shock,sepsis,and multiple organ dysfunction syndrome (MODS),and has become one of the most important causes of death for intensive care units (ICU) patients.Currently,although there has been some progress in knowledge ofThe occludin and ZO-1 complex,defined by small angle X Jul 03,2012·Y474 phosphorylation is associated with binding p85 of phosphatidyl inositol 3-kinase at the leading edge of migrating cells while S490 and S508 are associated with vascular permeability after VEGF treatment or HIV encephalitis .Our results suggest a roleVEGF activation of protein kinase C stimulates occludin VEGF-induced vascular permeability in vivo and in cell culture requires PKC activity,but the mechanism by which PKC regulates barrier properties remains unknown.This study was conducted to examine how VEGF and diabetes alter occludin phosphorylation and endothelial cell permeability.

William W Hauswirth Powell Gene Therapy Center

Occludin S490 Phosphorylation Regulates Vascular Endothelial Growth Factor-Induced Retinal Neovascularization.The American journal of pathology.186(9):2486-99 [DOI] 10.1016/j.ajpath.2016.04.018.[PMID] 27423695.ncbi.nlm.nih.govOccludin S490 Phosphorylation Regulates Vascular Cited by 15Publish Year 2016Author Xuwen Liu,Alyssa Dreffs,Monica Díaz-Coránguez,E.Aaron Runkle,Thomas W.Gardner,Vince A.ChiodoHerein,we demonstrate that occludin S490 phosphorylation also regulates VEGF-induced retinal endothelial cell proliferation and neovascularization.Using a specific antibody,phospho-occludin was located in centrosomes in endothelial cell cultures,animal models,and human surgical samples of retinal neovessels.Occludin S490 Phosphorylation Regulates Vascular Sep 01,2016·Vascular endothelial growth factor (VEGF) induces phosphorylation of occludin at S490,which is required for VEGF-induced endothelial permeability.Herein,we demonstrate that occludin S490 phosphorylation also regulates VEGF-induced retinal endothelial cell proliferation and neovascularization.


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